不同添加劑對紅三葉青貯品質(zhì)影響的研究

The Effect of Additives on Red Clover Silages Quality
TAO Lian1，YU Zhu1﹡，CHEN Yan1，YUN Peng2
（1.Institute of Grassland Science, China Agriculture University, Beijing 100094;2 .Animal Husbandry and Veterinary terminus in Beijing，Beijing 100029)
Abstract : The experiment was conducted to investigate the effects of different silage additives on fermentation quality and chemical composition of red clover silages. The silage additives include: FAST-SILE(FS), cellulase(CE), molasses（M）, formic acid(FA) . The results showed that The pH and the content of Non-protein Nitrogen in all the treatments were markedly lower than the control（p<0.05）. The lactic acid content of FAST-SILE treatment and FAST-SILE - cellulase treatment were significantly higher than the control（p<0.05）, and the content of the water soluble carbohydrates in the treatment of FAST-SILE - cellulase was markedly decreased(p<0.05). The content of  the water soluble carbohydrates of the treatments of formic acid were significantly increased (p<0.05) , and the content of Ammonia Nitrogen and lactic acid with formic acid  treatment were further decreased(p<0.05). Additives improved the fermentation quality of red clover silages and changed their chemical composition.
Key Words: Red clover; Silage; Additives; Fermentation quality; Water soluble carbohydrate

注：C：對照，control；FS：青寶II号，FAST-SILE；CE：纖維素酶，cellulase；M：糖蜜，molasses； FA：甲酸，formic acid；下表同, the same as below

注：表中同列不同小(xiǎo)寫字母表示平均數差異顯著（p<0.05） 下表同
Note: Different letters in the same column show significant differences at a 0.05 level，the same as below

2.4紅三葉青貯綜合評分(fēn)方案

3讨論

[1]  張文(wén)淑.紅三葉 [J].農村養殖技(jì )術,2002 ,15:27. 

[2]  楊淑玺.青貯飼料的制作(zuò)技(jì )術[J].農村經濟與科(kē)技(jì )，2004,15（6）：48

[3]  王晶.紅三葉與紫花(huā)苜蓿蛋白質(zhì)利用(yòng)率的比較[J].草(cǎo)業科(kē)學(xué)，2005,23（1）：52-54

[4]  Owens V N, Albrecht K A, Muck R E, Duke S H. Protein degradation and fermentation characteristics of red clover and alfalfa silage harvested with varying levels of total nonstructural carbohydrates[J].  Crop Science,  1999, 39: 1873-1880

[5]  張麗英. 飼料分(fēn)析及飼料質(zhì)量檢測技(jì )術[M]. 北京: 中國(guó)農業大學(xué)出版社, 2002：49-73

[6]  Y Zhu, N Nishino, Y Kishida, S Uchida. Ensiling characteristics and ruminal degradation of Italian ryegrass and Lucerne silages treated with cell wall-degrading enzymes[J]. Science,  1999, 79(14):1987-1992

[7]  許慶方.貯藏期和添加綠汁發酵液對袋裝(zhuāng)苜蓿青貯的影響[J].草(cǎo)業科(kē)學(xué)，2006，14(2):129-133

[8]  楊勝.飼料分(fēn)析及飼料質(zhì)量檢測技(jì )術[M].北京:中國(guó)農業大學(xué)出版社,1999,23-25

[9]  劉建新(xīn)，楊振海，葉均安(ān)，史占全，吳躍明 .青貯飼料的合理(lǐ)調制與質(zhì)量評定标準 [J]. 飼料工業 ，1999，20（4）:3-5

[10] Gordon F J. Improving the feeding value of silage through biological control [A]. Proceedings of the All-tech European Lecture Tour [C]. Birmingham, Alltech U K, 1992. 2-17.

[11] 時建忠.青貯接種菌的研究[J].中國(guó)飼料，2003，(5):15-17

[12] 彭海蘭.不同青貯添加劑對苜蓿青貯品質(zhì)的影響[D]：[學(xué)位論文(wén)].北京：中國(guó)農業大學(xué), 2003

[13] Selmer-olsen I. Enzymes as silage additives for grass-clove mixtures [J]. Grass and Forage Science,  1994,49(3)：305-315

[14] Jacobs J L and Mc Allan A B. Enzymes as silage additives for Silage quality, digestion,Digestibility and performance in growing cattle[J]. Grass and Forage Science, 1991,46(1)63-68

[15] Jaakkola S, Huhtanen P. The effect of cell wall degrading enzymes or formic acid on fermentation quality and on digestion of grass silage by cattle[J]. Grass and Forage Science, 1991, 46(1):75-80

[16] Sheperd A C , Kung L. Effect of an enzyme additive on composition of corn silage ensiled at various stages of maturity [J]. Dairy Science, 1996, 79:1767-1773

[17] M De Figueiredo, JP Marais. The effect of bacterial inoculants on Kikuyu silage quality[J]. Journal of the Agricultural science, 1994,122(11)53-60

[18] 玉柱，韓建國(guó)，胡躍高等.綠汁發酵液對豆科(kē)牧草(cǎo)青貯發酵品質(zhì)的影響[A].現代草(cǎo)業學(xué)進展-中國(guó)國(guó)際草(cǎo)業發展大會論文(wén)集[C],2002,159-162

[19] Ph illip L E. Effects treating lucerne with an inoculum of lacticacid  bacteria  or 

formic acid upon chemical changes during fermen2tation, and upon the nutritive value of the silage fo r lambs [J ].Grass and Forage Science, 1990, (45) : 337～ 344

[20] Chamberlain D G. The rate of addition of formic acid to grass at ensilage and the subsequent digestion of the silage in the rumen and intestine of sheep [J ]. Grass and Fo rage Science, 1982, (37) :159～ 164

[21] Alexander N Hristov, Sasho G Sandev. Proteolysis and rumen degradability of protein in alfalfa preserved as silage, wilted silage or hay[J]. Animal Feed Science Technology,1998,72:175-181